A color filter substrate, a manufacturing method thereof, and related devices. The color filter substrate includes: a base plate; a color filter layer on the base plate; and a transparent layer on a side of the color filter layer away from the base plate. Specifically, the color filter layer includes multiple rows of color resist units, wherein color resist units in each row have at least three different colors. In addition, a first distance between a surface of the base plate close to the color resist units and a surface of the transparent layer away from the color resist units may be different for color resist units of different colors.