A system and method for automating a recorded observed behavior of a laboratory animal in a clinical trial is disclosed. The present invention records a video of the laboratory animal with respect to one or more objects. The recorded data is utilized to detect at least one feature points such as head, whiskers, tail, waist etc. of the animal and a one or more objects in video, wherein the one or more objects are a known object and an unknown object. The present invention thus automatically determines the behavior characteristics of the animal by measuring a proximity and comparing the proximity with a predefined threshold criterion with respect to the one or more objects without human intervention. The behavior characteristics are determined in the form of a type of behavioral events, a time interval, number of occurrences of each type of behavioral events, latency, and distance travelled by the animal.