The present application provides a display panel motherboard and a method of manufacturing a display panel motherboard. The display panel motherboard includes a substrate; a plurality of display panels formed on the substrate; cutting grooves formed between front process layers of the plurality of display panels, wherein the cutting grooves comprise a first cutting groove and a second cutting groove, and an orthographic projection of the second cutting groove on the substrate covers and is larger than an orthographic projection of the first cutting groove on the substrate. The present application guarantees an effective area of package.