Disclosed is a method for determining internal uniaxial stress of steel members based on transverse wave phase spectrum, including: manufacturing a replicated steel member of an in-service steel structure member, where the replicated steel member and the in-service steel structure member are the same in material and thickness; loading a test on the replicated steel member to obtain two stress-spectral parameters; performing ultrasonic determination on the in-service steel structure member using an ultrasonic determination device; and collecting transverse wave signals using a signal acquisition system; processing the collected transverse wave signals through an information processing device to obtain a derived curve of the phase spectrum; capturing a first response frequency of the phase spectrum from the phase spectrum derived curve; and obtaining a uniaxial stress of the in-service steel structure member according to the stress-spectral parameters.