A stuffing filling and gas charging system of a dough food product machine is arranged between a stuffing feeding unit and a dough feeding unit of the dough food product machine and includes a stuffing conveyance pipe, and a shaft sleeve, a dough driving rod, and a screw rod support enclosure sleeved over the stuffing conveyance pipe. A first passage is formed between the shaft sleeve and the stuffing conveyance pipe; a second passage is formed between the dough driving rod and the stuffing conveyance pipe; and a third passage is formed between the screw rod support enclosure and the stuffing conveyance pipe. The passages are in communication with each other and are connected to a gas supply unit, so that air flow is introduced into a dough skin during the dough skin being shaped to prevent the dough skin from wrinkling and collapsing before being properly shaped.