This disclosure relates to systems and methods for dynamically mapping content based on information relating to a user. In various embodiments, a segment associated with a user may be identified, and a content service may map the presentation of advertisements and/or other content within rendered content in a manner that is tailored to the identified segment. In this manner, advertisement and/or other content impressions may be targeted to the user based on their identified segment. Further embodiments facilitate defining and refining content maps for a particular segment until a content mapping meeting certain objective criteria is realized.