A method of is provided as a process of substrate lift-off. The present invention is mainly used for a group III-V solar cell, which has the highest power generation efficiency. An original sacrificial layer is changed into an AlAs oxide layer, which is transformed into an AlOx sacrificial layer after wet oxidation. The sacrificial layer is then soaked in an oxide-relief solution for etching. Thus, the lift-off process of a GaAs substrate can be harmlessly processed to the complex group III-V solar cell. The GaAs substrate can be recycled to be effectively further reused in photovoltaic devices with reduced cost.