Provided is a wireless positioning calibration system, including a plurality of transmission base stations, at least one sniffer base station and a positioning server. The at least one sniffer base station receives a plurality of channel state information (CSI) transmitted by the plurality of transmission base stations. The positioning server receives the plurality of CSI transmitted by the at least one sniffer base station. The positioning server calculates a phase error and an antenna spacing error generated by the at least one sniffer base station by means of the plurality of CSI, and compensates the phase error and the antenna spacing error. A wireless positioning calibration method is also provided.