Pipette tip transfer devices allow for the pick-up of pipette tips from one location and the transfer the pipette tips to another location where the tips can then be released. The pipette transfer device includes a strip plate having a linear array of apertures positioned at a lower end of a frame. An actuator bracket is positioned within and mounted to the frame for reciprocal movements between lowered and raised positions, the actuator bracket carrying a linear array of pick-up pins dependently extending therefrom with each pin being received within a respective aperture of the strip plate. An actuator lock is mounted to the frame for manual manipulations between a locked position wherein movement of the actuator bracket is prevented when in the lowered position to allow frictional engagement between and pick-up of the pipette tips by the pins, and a released position whereby movement of the actuator bracket into the raised position thereof is allowed to thereby strip the frictionally engaged pipette tips from the pins.