An active noise cancellation device having an acoustic filter includes: a casing, an active noise cancellation unit, a speaker, a noise cancellation processor and an acoustic filter. The casing includes a first channel and a second channel, wherein a channel length of the first channel is greater than a channel length of the second channel. The active noise cancellation unit includes an external microphone disposed outside the casing. The active noise cancellation is configured to detect an ambient noise, wherein a location of the external microphone corresponds to a location of the first external end of the first channel. The speaker is disposed at a second external end of the second channel, and is configured to output a phase-inverted signal of the ambient noise. The noise cancellation processor electrically couples with the external microphone and the speaker. The acoustic filter is disposed inside the first channel.