Systems and methods are provided for determining operation of an autonomous vehicle based on user profiles. In one example, a computer-implemented method of applying user profiles for autonomous vehicle operation is provided. The method includes receiving, by a computing system comprising one or more computing devices, a trip request from a user; retrieving, by the computing system, a user profile associated with the user; determining, by the computing system, one or more trip attribute settings based at least in part on the user profile; and providing, by the computing system, the determined one or more trip attribute settings to a vehicle computing system for determining autonomous vehicle operation during a trip associated with the trip request.