An image pickup apparatus, includes a first optical system and a second optical system which generate two optical images having mutual parallax; and one image sensor which captures the two optical images, wherein each of the first optical system and the second optical system has a different focusing unit, further includes a first frame which holds some of lenses in the first optical system, a second frame which holds the image sensor; and a third frame which holds lenses in the first optical system other than the lenses held by the first frame, and lenses in the second optical system, wherein the first frame is movable in a direction of optical axes with respect to the third frame, and the second frame is movable in a direction of optical axes with respect to the third frame.