Invention Grant
- Patent Title: Recombinant DNA polymerase for improved incorporation of nucleotide analogues
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Application No.: US15798846Application Date: 2017-10-31
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Publication No.: US10662413B2Publication Date: 2020-05-26
- Inventor: Shiuan-Woei Lin Wu , Ching-Wei Tsai , Ting-Yueh Tsai , Jyun-Yuan Huang , Chao-Chi Pan , Li-Chi Chang , Ching-Long Hwong
- Applicant: PERSONAL GENOMICS, INC.
- Applicant Address: KY Grand Cayman
- Assignee: PERSONAL GENOMICS, INC.
- Current Assignee: PERSONAL GENOMICS, INC.
- Current Assignee Address: KY Grand Cayman
- Agency: Muncy, Geissler, Olds & Lowe, P.C.
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12P19/34 ; C12P19/30

Abstract:
A composition comprising a recombinant DNA polymerase and 3′-esterified nucleotide analogues is provided. The recombinant DNA polymerase includes an amino acid sequence that is at least 90% homology with 9° N DNA polymerase (SEQ ID NO: 1), and the recombinant DNA polymerase includes at least two mutations at the positions corresponding to amino acid residues 141 and 143 of the 9° N DNA polymerase. A recombinant DNA polymerase is further provided. The recombinant DNA polymerase includes an amino acid sequence that is at least 90% homology with 9° N-III DNA polymerase (SEQ ID NO: 3), and the recombinant DNA polymerase includes one or two mutations at the positions corresponding to amino acid residue 480 and 486 of the 9° N-III DNA polymerase. A method of performing a polymerization reaction is also provided.
Public/Granted literature
- US20180119115A1 RECOMBINANT DNA POLYMERASE FOR IMPROVED INCORPORATION OF NUCLEOTIDE ANALOGUES Public/Granted day:2018-05-03
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