The present disclosure provides a display screen, a terminal display screen assembly, and a mobile terminal. The display screen defines a first through hole which penetrates the display screen in a thickness direction of the display screen, and may include: a frame, a display module and a positioning structure. The frame may include a first face and a second face opposite to the first face. The display module may be arranged on the first face of the frame. The positioning structure may be arranged on the second face of the frame. The first through hole may include a first sub-through hole defined in the display module and penetrating the display module in a thickness direction of the display module, and a second sub-through hole defined in the frame and penetrating the frame in a thickness direction of the frame and communicating to the first sub-through hole. The positioning structure is disposed to surround the second sub-through hole.