An array substrate and a display device are provided. The array substrate includes a base substrate; a first active layer, located on the base substrate; a first insulating layer, located on the first active layer and the base substrate; a gate electrode, located at a side of the first insulating layer away from the first active layer; a second insulating layer, located on the gate electrode and the first insulating layer; a second active layer, located on the second insulating layer away from the gate electrode; a first drain electrode and a first source electrode, being in partial contact with the first active layer, respectively; a second drain electrode and a second source electrode, being in partial contact with the second active layer, respectively; and a pixel electrode, the first drain electrode and the second drain electrode are electrically connected, the first source electrode and the second source electrode are electrically connected, and the pixel electrode is electrically connected with at least one of the first drain electrode and the second drain electrode. The array substrate can improve the responding speed and charging efficiency, and avoid increasing the aperture opening ratio at the same time.