The present disclosure relates to systems and methods for image splicing. The systems and methods may acquire a first image and a second image, determine a plurality of first feature points in a first region of the first image, determine a plurality of second feature points in a second region of the second image, then match the plurality of first feature points with the plurality of second feature points to generate a plurality of point pairs. Based on the plurality of point pairs, a third region on the first image and a fourth region on the second image may be determined. Finally, a third image may be generated based on the first image and the second image, wherein the third region of the first image may overlap with the fourth region of the second image in the third image.