A method of desalinating mura and dot/line defects of a display panel is provided, including the following steps: S1: providing a display panel; S2: making the display panel display an alignment mark image; S3: accurately aligning an screen detector with the display panel; S4: photographing and recording the display panel to obtain a display image of the display panel; S5: extracting the display image and calculating a compensation image; S6: inputting the compensation image into a printer to generate a compensation film; S7: accurately attaching the compensating film on the display panel. Compared with the related art, in the method, by performing observation of lighting-on the display panel, recording the defect position, and printing the compensation film by thin film printing and precisely attaching the compensation film on the display panel, the display status is improved, and the problem of the mura and the dot/line defects is solved.