An adjustment device for light-on testing and a light-on testing device are provided. The adjustment device for light-on testing including a substrate, a first pressing member and a second pressing member disposed on the substrate, wherein, the first pressing member and the second pressing member are disposed on the substrate and capable of moving with respect to each other, so that a distance therebetween is adjustable; and the first pressing member and the second pressing member are respectively provided with a probe for contacting a product to be subjected to the light-on testing.