A mask plate, a method for manufacturing the mask plate and a usage of the mask plate in manufacturing a display substrate are provided. The mask plate includes a transparent substrate and at least two layers of electrochromic thin film patterns arranged on the transparent substrate. Each of the at least two layers of electrochromic thin film patterns is configured to be capable of being switched between a light-transmissible state and a non-light-transmissible state under an effect of an electric field. The mask plate is capable of forming at least two different mask patterns on the display substrate, which reduces the number of mask plates required for manufacturing the display substrate.