A slicing machine having a cutting head and a knife assembly mounted thereto, and methods for clamping a knife of the knife assembly in the slicing machine. The knife assembly includes a knife holder having a support surface that supports the knife and a clamp overlying the knife holder so that the knife is between the support surface of the knife holder and the clamp. A clamping rod is rotated from a release position to a clamping position to secure the clamp to the knife holder and clamp the knife therebetween. The clamping rod is eccentrically mounted to the cutting head and uses eccentricity to apply a clamping load to the clamp when rotated from the release position to the clamping position.