Disclosed are a pixel structure, a display panel and a driving method thereof, the pixel structure comprising a plurality of alternately arranged first sub-pixel columns and second sub-pixel columns. The first sub-pixel columns comprise sub-pixels of two colors, the second sub-pixel columns are sub-pixel columns of single color, and the number of the first sub-pixel columns is one larger than that of the second sub-pixel columns. The first sub-pixel columns are one column more than the second sub-pixel columns, such that the whole pixel arrangement is a symmetrical arrangement.Therefore, when in mirror display, an image signal processing algorithm consistent with that in normal display can still be maintained.