Systems and methods for adjusting for non-invasive measurement of biological analytes. Exemplary methods include: illuminating an analyte using first light, the first light having a first excitation wavelength; detecting a first spectrum from the analyte illuminated by the first light, the first spectrum including a first Raman signal and fluorescence; illuminating the analyte using second light, the second light having a second excitation wavelength; detecting a second spectrum; illuminating the analyte using third light, the third light having a third excitation wavelength; detecting a third spectrum; recovering the first Raman signal using the first spectrum, the second spectrum, and the third spectrum using an inverse transform; and using the first Raman signal to identify and measure at least one molecule of the analyte using a database of identified Raman signals.