The present disclosure provides a method and a device for inspecting a defect of a liquid crystal panel. The method includes steps of: performing AOI treatment on an image of the liquid crystal panel; determining a plurality of to-be-treated targets that do not meet a defect specification requirement in accordance with an AOI treatment result; ranking the plurality of to-be-treated targets in a predetermined order, and acquiring an aggregation degree parameter of every two adjacent to-be-treated targets of the ranked to-be-treated targets; comparing each aggregation degree parameter with a predetermined aggregation degree parameter to acquire a plurality of comparison results; and determining, in accordance with the plurality of comparison results, the to-be-treated targets corresponding to n consecutive aggregation degree parameters smaller than the predetermined aggregation degree parameter as aggregate-type defects of the liquid crystal panel, n being a natural number and greater than or equal to a predetermined value.