Methods and systems for determining permeability, as a function of pore pressure, and porosity of a subsurface formation. The method includes positioning a sample in a sample assembly comprising of a gas and a pressure gauge, inside a pressure vessel comprising gas or liquid and a pressure gauge, measuring a first gas pressure, pi, of the sample inside the pressure vessel, applying a second gas pressure, po, to the pressure vessel, the second gas pressure being greater than the first gas pressure, measuring a third gas pressure, p, at time, t, at location, x, from the inlet of sample inside the pressure vessel, determining a total gas mass per unit volume of the subsurface formation, m, and determining the permeability, k, of the subsurface formation as a function of pore pressure based at least in part on the first gas pressure, the second pressure, the third gas pressure, and the gas density, with a single test run.