An image capturing apparatus comprising: an image sensor having a plurality of photoelectric conversion portions that correspond to each of a plurality of microlenses arranged in a matrix; a control circuit that controls read-out from the image sensor by either of first read-out control for obtaining focus detection signals and second read-out control for obtaining an image signal, a setting circuit that sets rows to be read out by the first read-out control among rows that include a focus detection area; an amplification circuit that amplifies a signal with a gain set in accordance with an exposure state; and a signal processing circuit that performs signal processing on an image signal using an image signal of neighboring rows, wherein the setting circuit sets the rows to be read out by the first read-out control according to the gain.