Methods, systems, and computer readable media for emulating network traffic patterns on a virtual machine are disclosed. In one example, the method includes generating a user specification based on a plurality of network traffic patterns and computer processing usage patterns and receiving, at a virtual machine hosted by a DUT, the user specification containing the plurality of network traffic patterns and computer processing usage patterns. The method further includes generating a plurality of network traffic sequences based on the plurality of network traffic patterns and a plurality of computer processing usage pattern instruction sequences based on the plurality of computer processing usage patterns. The method also includes distributing each of the plurality of network traffic sequences among a plurality of virtual network interface cards (VNICs) and distributing each of the plurality of computer processing usage pattern instruction sequences among a plurality of virtual processing cores in the virtual machine.