A display panel and a manufacturing method thereof are provided. The display panel includes a color resist layer, a protective layer and a light-shielding layer. The color resist layer is formed with a first channel having a first color resist block and a second color resist block therein. The light-shielding layer has a base, a first protrusion corresponding to the first color resist block, and a second protrusion corresponding to the second color resist block. The first protrusion is configured as a main photo spacer. The second protrusion is configured as a sub photo spacer. The protective layer is formed with a second channel corresponding to the second color resist block. The sub photo spacer is directly connected to the second color resist block.