Disclosed are a manufacture method of quantum dots printing ink and manufactured quantum dots printing ink manufactured with the manufacture method. In the manufacture method, by mixing the first solvent and the second solvent, the viscosity of the quantum dots printing ink is adjusted to be in a predetermined range. Then, by mixing the third solvent with the first and second solvent, the surface tension thereof is adjusted to be in a predetermined range while maintaining the viscosity to be in the predetermined range. Furthermore, by adding the fourth solvent in the quantum dots printing ink, the vapor pressure is adjusted to be in a reasonable range. With mixture of kinds of solvents, the quantum dots printing ink of which the performance indicators, the viscosity, the surface tension, the dry condition are suitable for jet ink printing can be formulated to avoid adding the surfactant in the ink.