The present application discloses a detection system for image sticking of display panel and method thereof, includes obtaining the display panel image to be detecting the image sticking; obtaining the image display portion of the display panel in the display panel image; performing Fourier transform to the image display portion, obtaining a first spectral energy value with characteristic frequency corresponding to the image sticking in the image display portion, and a second spectral energy value of the reference frequency in the image display portion; determining the ratio of the first spectral energy value and the second spectral energy value is exceeding the numerical range of the corresponded qualified detection, if beyond, it is judged as disqualified. By the method described above, the present application can solve the conventional image sticking judgment based on human subjectivity, accurate judgment and objectively evaluation of severity the image sticking of the display panel.