A symbol rate estimating device includes: a power spectrum density (PSD) estimating unit, estimating a PSD of an input signal; an index searching unit, searching for a cut-off frequency index in the PSD; an adjacent channel interference (ACI) detecting unit, detecting whether the input signal has ACI to generate a detection signal; a threshold adjusting unit, generating an adjusted index number threshold according to the detection signal; an index output unit, outputting the cut-off frequency index according to the adjusted index number threshold; and a symbol calculating unit, calculating a symbol rate of the input signal according to the cut-off frequency index.