A fluorescent image acquisition system includes a light emitting device which emits excitation light during ON period and stop the emission of the excitation light during OFF period, an imaging device which outputs ON image data and OFF image data, an input device which sets a coefficient, an image processing unit which creates fluorescent image data and background image data, and an identification unit which identifies fluorescent pixels and non-fluorescent pixels. In order to create a superimposed image, the image processing unit calculates values obtained by summing pixel values of fluorescent pixels of the fluorescent image data and pixel values of fluorescent pixels of the background image data at a ratio of 1:1, as pixel values of the fluorescent pixels and calculates values obtained by multiplying at least pixel values of the non-fluorescent pixels of the background image by the coefficient, as pixel values of the non-fluorescent pixels.