Embodiments of the present disclosure provide a temperature compensation circuit, a display panel and a temperature compensation method. The temperature compensation circuit comprises: a temperature sensing unit, adapted to sense a temperature of an external environment; a temperature compensation control unit being adapted to compare the temperature sensed output voltage with a reference voltage, and generate a control signal based on the comparison result; and a first voltage source adapted to receive a control signal from the temperature compensation control unit, generate a corresponding driving voltage based on the control signal and output the corresponding driving voltage to a gate drive circuit as a gate driving voltage of the gate drive circuit, and generate a feedback signal according to the control signal and output the feedback signal to the temperature sensing unit and the temperature compensation control unit, the reference voltage being variable based on the feedback signal.