The present invention discloses a method for acquiring a comfort degree of a motion-sensing binocular stereoscopic video. The method includes the following steps: S1. carrying out binocular parallax and movement speed analysis on an input binocular video, and calculating a binocular parallax d, a movement speed vxy in a visual plane direction and a movement speed vz in a depth direction of each pixel point in the video; S2. calculating a corresponding comfort degree value c according to the parameters (d, vxy, vz) of the pixel point; S3. calculating the comfort degree value of the entire frame according to the calculated comfort degree value of each pixel point; S4. calculating the comfort degree value of the entire video according to the comfort degree value of each frame. The comfort degree of the binocular stereoscopic video is calculated according to the steps in the present invention, so that influence of positive and negative depth ranges and object movement on the comfort degree can be well considered to obtain more accurate estimation of the comfort degree.