The utility model discloses a bidirectional detection device for clenbuterol and malachite green; the bidirectional detection device is characterized by comprising a base, test paper in the base and an upper cover connected to the base; the base is of an oblong, which is arc-shaped at the left end; a test paper groove for holding the test paper is arranged inside the base; a test paper isolator is arranged in the middle of the test paper groove; the test paper is of a U-shaped structure; a sample pad is arranged at the bottom of the U-shaped structure; the sample pad is divided by a test paper isolation area into a first sample pad and a second sample pad; a clenbuterol detection area corresponding to the first sample pad and a malachite green detection area corresponding to the second sample pad are formed; the upper cover is tallied with the base; and a sample dripping window is arranged at the left end of the upper cover. The bidirectional detection device for clenbuterol and malachite green is capable of realizing bidirectional detection for clenbuterol and malachite green and is simple in structure; the test paper is sealed by the glass base and the upper cover, is convenient to store, is prevented from being polluted by the outside and is convenient for detection personnel to use and observe and suitable for professionals and ordinary consumers to use.