The utility model relates to a three-channel percutaneous puncture vessel cannula which comprises a multi-channel cock 3, wherein the multi-channel cock 3 and a puncture needle 1 are connected at two ends of a hose 2. According to the three-channel percutaneous puncture vessel cannula, two or more than two channels can be simultaneously opened for simultaneous input of two different liquid, or one channel is used for inputting a liquid, and the other channel is used for connecting with a sensor of an experimental facility, so that changes of experimental indexes can be recorded in real time when experimental intervention is provided. The three-channel percutaneous puncture vessel cannula has a simple structure, is convenient to use and operate, can not be destroyed easily, and can be used repeatedly after high-pressure sterilization.