The invention provides a preparing method of a high-strength and high-conductivity copper wire. The preparing method comprises the steps of S1, molten copper preparing; S2, alloy element adding; S3, copper rod casting; S4, copper busbar preparing; and S5, high-strength and high-conductivity copper wire preparing. The preparing method has the advantages of being excellent in electric conductivity,tensile strength, high temperature resistance, wear resistance, continuity and stability.