The invention relates to an electronic beam cold bed furnace water cooling copper crystallizer and a titanium alloy preparation method, and belongs to the technical field of alloy material preparation. The electronic beam cold bed furnace water cooling copper crystallizer comprises a crystallizer and an ingot pulling mechanism; the crystallizer comprises a crystallizer body, an upper pouring gate,a water cooling outer shell and a fixed plate; the crystallizer body is arranged in the water cooling outer shell; a crystallization cavity is vertically formed in the crystallizer body; a cavity formed between the crystallizer body and the water cooling outer shell is a cooling cavity; the upper pouring gate is arranged at the top of the crystallizer body; the fixed plate is fixedly arranged atthe bottom of the water cooling outer shell; and the ingot pulling mechanism is inserted from the bottom into the crystallization cavity in the crystallizer body, and can move up and down in the crystallization cavity of the crystallizer body. The electronic beam cold bed furnace water cooling copper crystallizer is simple in structure, convenient to operate, uniform in cooling of cast blanks, high in quality of produced products and high in material forming rate.