The invention discloses a preparation method of a mold-proof isolation material for solar calendaring glass, which belongs to the technical field of refining chemical industry. The preparation methodcomprises the following steps: stirring and mixing the primarily processed sugarcane residues and ethyl orthosilicate, filtering, washing, drying, reacting at a high temperature, calcining, and obtaining secondarily processed sugarcane residues; stirring and mixing the secondarily processed sugarcane residues and a dopamine solution, filtering, and obtaining a tertiarily processed sugarcane residues; stirring and mixing the tertiarily processed sugarcane residues and activated chitosan solution, filtering, drying, carbonizing, and obtaining the quaternarily processed sugarcane residues; mixingand soaking the quaternarily processed sugarcane residues and hydrofluoric acid, filtering, washing, drying, and obtaining modified sugarcane residues; and mixing the modified sugarcane residues, modified additives, animal oil, resin particles and phospholipid, sieving, and obtaining the mold-proof isolation material for the solar calendaring glass. The mold-proof isolation material for the solarcalendaring glass has excellent mold-proof performance and mobility.