The invention discloses a no-wash dyeing process of skein. The no-wash dyeing process comprises the following steps: S1, soaking the skein into water at room temperature; S2, adding a no-wash agent into the water after 10 to 20 minutes, wherein the adding amount of the no-wash agent is 1 to 2 g/L of water; S3, adding organic acid into the water after 5 to 10 minutes, wherein the adding amount of the organic acid is 2 to 4g/L of water; S4, adding a levelling agent into the water after 5 to 10 minutes, wherein the adding amount of the levelling agent is 1 to 2g/L of water; S5, adding dye into the water after 10 to 20 minutes; S6, raising the temperature from the room temperature to 95 to 100 DEG C according to the speed of 1 to 2 DEG C/min and carrying out thermal insulation for 30 to 60 minutes; cooling to room temperature and taking out the skein to obtain the dyed skein. According to the no-wash dyeing process disclosed by the invention, by improving the dyeing process and omitting the pretreatment (washing line) procedure, energies of human power, materials and financial resources, especially water, electricity and gas are saved and a great promoting effect on energy conservationand emission reduction is achieved; the work efficiency is improved and the production cost is reduced due to the reduction of the process flow.