The invention discloses a green and environmentally-friendly air purifying anti-bacterial anti-skid automobile floor mat and a preparation method thereof. The green and environmentally-friendly air purifying anti-bacterial anti-skid automobile floor mat comprises a loofah sponge purifying layer, a carbon absorption layer and a base material layer in sequence from top to bottom, wherein an antibacterial agent and a photocatalyst auxiliary are supported on the loofah sponge purifying layer; the carbon absorption layer is a biomass base multi-pore carbon fiber; and the base material layer is an ultra-high molecular weight polyethylene/linen fiber layer. The green and environmentally-friendly air purifying anti-bacterial anti-skid automobile floor mat has the advantages that the process is simple, large-scale production can be realized, good application prospect is realized, the mat is soft and comfortable, the mat is anti-bacterial, the dust is suppressed, and the air in an automobile ispurified; and by using a biomass material, the green and environmentally-friendly air purifying anti-bacterial anti-skid automobile floor mat is green and environmentally-friendly, and wastes are recyclable and degradable.