The invention discloses an antireflection film capable of filtering out blue light and a preparation method thereof. Specifically, the antireflection film capable of filtering out the blue light is formed on a transparent substrate, wherein the antireflection film is sequentially provided with a ZnxCd1-xO film layer, a Al2O3 film layer, a ZnxCd1-xO film layer and a Al2O3 film layer from the innersurface of the substrate to the exterior of the substrate. According to the preparation method of the antireflection film capable of filtering out the blue light, during magnetron sputtering, according to actual application demands, antireflection films capable of filtering out the blue light which are different in absorption edge wavelength lambda can be prepared by adjusting Cd components x in the ZnxCd1-xO film layers. The average absorption rate of the antireflection film capable of filtering out the blue light on the blue light within the wave band range from 300 nm to (lambda-15) nm is higher than 99%, and the average transmissivity of the antireflection film on visible light within the wave band range from (lambda+15) nm to 780 nm is higher than 95%. A designed film system structureof the antireflection film is simple, a process is easily implemented, and industrial production can be achieved.