The invention discloses a manufacturing mold, manufacturing equipment and manufacturing method of a Fresnel microstructure, and relates to the technical field of laser projection display. The manufacturing mold, manufacturing equipment and manufacturing method can solve the problem of a present manufacturing method that the production efficiency of a circular Fresnel anti-light screen is low due to the failure of continuous production. The manufacturing mold of the Fresnel microstructure includes a rolling member. The rolling surface of the rolling member is a cylindrical surface. The cylindrical surface is provided with multiple strip-shaped extrusion structures. The extrusion structures are arranged around the cylindrical surface. The multiple extrusion structures are arranged along theaxis of the rolling element. The sections, being on the axis direction of the rolling element, of the multiple extrusion structures are spliced into a zigzag shape. The multiple extrusion structures are arc-shaped when the cylindrical surface is expanded into a rectangular plane. The manufacturing mold, manufacturing equipment and manufacturing method are used for manufacturing the Fresnel microstructure.