The invention relates to the field of preparation of (Cu,Fe)3O4 spinel coatings, in particular to a method for preparing a (Cu,Fe)3O4 spinel coating through a magnetron sputtering method and an application thereof. The method includes the following specific preparation steps that (1) pretreatment and pre-oxidation treatment are carried out; (2) sputtering deposition is carried out to obtain an alloy coating; and (3) thermal inversion is carried out to obtain the spinel coating. The method has the characteristics that the operation process is simple and the deposition speed is high, and the prepared spinel coating is compact, has good binding force with a substrate, has excellent long-time resistance to high temperature oxidation and conductivity, and can effectively suppress external diffusion of substrate Cr.