The invention discloses an anti-static tatami. The anti-static tatami comprises a cushion main body; the cushion main body comprises an upper surface fabric layer, a middle core material, and a lowersurface antiskid cloth; the upper surface fabric layer is prepared via weaving of warp and weft; an anti-static agent is added into the weft; the anti-static agent comprises, by weight, 50 to 65 partsof Hexadecyltrimethylammonium chloride, 20 to 30 parts of nanometer silver oxide, 5 to 10 parts of polyether ester amide, 2 to 5 parts of a poly(ether-ester), 1 to 5 parts of silane coupling agent, 1to 5 parts of poly(ethylene oxide), and 2 to 3 parts of carbon black. In preparation process, modification on the warp and the weft of the upper surface fabric layer is carried out so that the uppersurface fabric layer possesses certain anti-static performance; and at the same time, compared with latex cushions taking natural latex and artificial latex as the main raw material, the elasticity ofthe anti-static tatami is relatively high.