The invention provides a vincristine drug resistance-related microRNA (Ribonucleic Acid) molecule miR-6847-5p and applications thereof and belongs to the technical fields of gene engineering and tumor biotherapy. The nucleotide sequence of the miR-6847-5p is shown as SEQ ID NO.1; the miR-6847-5p has the characteristics of high expression in vincristine drug-resistant cells and low expression in vincristine-sensitive cell lines and can serve as a vincristine drug-resistant marker to be used for preparing vincristine drug resistance auxiliary diagnostic kits aiming at colon cancer; and a new target is provided for designing vincristine drug resistance drugs of the colon cancer. The invention provides the applications of the miRNA marker to preparing the vincristine drug resistance auxiliary diagnostic kits used for treating the colon cancer and screening new drugs for treating the colon cancer and the application of a miRNA inhibitor to preparing vincristine drug-resistance resisting drugs; and the miR-6847-5p has a good clinical application value.