The invention belongs to the field of medicinal chemistry, and particularly relates to a new use and preparation method of a compound, namely the new use and preparation method of (3beta,5alpha,22alpha,25R)-spirosolane-3-O-beta-D-glucopyranosyl-(1->2)-O-[beta-D-xylopyranose-(1->3)]-O-beta-D-glucopyranosyl-(1->4)-beta-D-pyran galactoside; the compound can inhibit lung cancer cells, makes the growth period of the lung cancer cells arrested in the early stage and promotes apoptosis of the lung cancer cells. Therefore, the compound can be used in preparation of anticancer drugs, especially in preparation of anti-lung cancer drugs.