The invention relates to the technical field of pre-stress, particularly relates to the technical field of bridge engineering, and particularly relates to a pre-stressed steel strand pulling fixture and a method for operating the same. The pre-stressed steel strand pulling fixture comprises a steel strand pulling fixture. A barrel wall of the steel strand pulling fixture comprises a clip section, a slide rail section, a deformation section, a key slot section and a gathering section which are sequentially connected with one another, teeth, slide rails, umbrella-shaped gathering controllers and a gathering control shaft are arranged inside the steel strand pulling fixture, the teeth are arranged on the inner wall of the clip section, the slide rails are arranged on the inner wall of the slide rail section, one end of each umbrella-shaped gathering controller corresponds to the corresponding slide rail, the other end of each umbrella-shaped gathering controller is fixedly connected with a shaft lever of the gathering control shaft, and the distances from the slide rails to the slide rail section of the barrel wall are gradually increased from the clip section to the deformation section. The pre-stressed steel strand pulling fixture with the structure and the method have the advantages that the gathering control shaft can be hammered, so that clamping work can be carried out on steel strand harnesses by the clip section; a wedge-shaped steel plate can be inserted into a key slot of the barrel wall, can be inserted into a gathering control shaft key slot on the gathering control shaft and can be hammered, so that loosening work can be carried out on the steel strand harnesses by the clip section.