The invention discloses a pilot scale production process and production line of white zein. According to the production process, corn protein powder and ethanol water solution are mixed; then, dynamic multi-stage continuous reverse flow ultrasonic extraction is performed; extracts are firstly filtered by a scraper type filter to remove large particle impurity ingredients such as corn protein powder in the extraction solution, are then filtered by a ceramic membrane to remove filter slag of pectin fiber and the like, are next filtered through an ultra-nanofiltration membrane so that micromolecular substances such as maize yellow pigments can be separated from the zein; the zein solution is concentrated through the ultra-nanofiltration membrane, and ethanol solvents are recovered; next, vacuum concentration condensation is performed for recovering the ethanol for a second time; meanwhile, water is added for precipitating and separating out zein wet powder; the zein wet powder is dried and crushed to obtain the white zein product. The produced white zein has high quality, no solvent residue and green and health effects; the added value of the corn protein powder is improved; the integral development and utilization of the corn protein powder are facilitated.