The invention provides a detection method of wireless sensor network routing layer low-speed flooding attack. By the method, limited calculation and storage resources of wireless sensor network nodes can be saved, and nodes generating low-speed flooding attack traffic can be acquired. The method includes: monitoring routing packet traffic of a wireless network routing layer within a wireless network node communication range; offline detecting whether the monitored routing packet traffic contains the low-speed flooding attack traffic or not through a time domain-frequency domain combined analysis method based on Hilbert-Huang transformation; if yes, acquiring the nodes generating the low-speed flooding attack traffic according to the low-speed flooding attack traffic contained in the routing packet traffic. The detection method is suitable for the technical field of communication.