The invention discloses a neolignan compound for the treatment of gastric cancer, belongs to the pharmaceutical field, and in particular relates to a neolignan compound isolated from lignum millettiae for the treatment of gastric cancer, and a preparation method and pharmaceutical application thereof. The compound is reported for the first time, can be extracted, separated and purified from lignum millettiae and has high purity. In vitro tests prove that the compound can inhibit the proliferation of gastric cancer cells, can perform inhibitory activity individually and can also be combined with other anticancer drugs to enhance inhibitory activity; therefore, the compound can be developed and prepared into drugs for the treatment of gastric cancer.